细胞分析技术常用试剂及来源
类型 | 试剂名称 | 货号 | 用途 | 来源 | 使用比例 |
Alexa 350 | Goat anti-Mouse | A21049 | 免疫荧光二抗 | Molecular Probes | 1∶100 |
| Goat anti-Rabbit | A21068 | 免疫荧光二抗 | Molecular Probes | 1∶100 |
Alexa 488 | Goat anti-Mouse | A11029 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
| Goat anti-Rabbit | A11034 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
| Doney anti Goat | A11055 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
| Doney anti Mouse | A21202 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
| Doney anti Rat | A21208 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
| Donkey anti-Rabbit | A21206 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
Atto 550 | Goat anti-Mouse | 43394 | 免疫荧光二抗 | Sigma | 1∶1000 |
Alexa 568 | Goat anti-Mouse | A11031 | 免疫荧光二抗 | Molecular Probes | 1∶500-1000 |
Cy3 | Goat anti-Rabbit | 111-165-045 | 免疫荧光二抗 | Jackson | 1∶1000 |
| Donkey anti-Rabbit | 711-165-152 | 免疫荧光二抗 | Jackson | 1∶1000 |
| Goat anti-Rat | 112-165-167 | 免疫荧光二抗 | Jackson | 1∶1000 |
| Donkey anti-Goat | 705-165-147 | 免疫荧光二抗 | Jackson | 1∶1000 |
| Donkey anti-Rat | 712-165-150 | 免疫荧光二抗 | Jackson | 1∶1000 |
PE | Goat anti-mouse | 550589 | 免疫荧光二抗 | BD | |
Cy5 | Goat anti-Mouse | 115-175-146 | 免疫荧光二抗 | Jackson | 1∶500-1000 |
| Goat anti-Rabbit | 111-175-144 | 免疫荧光二抗 | Jackson | 1∶500-1000 |
| Donkey anti-Rabbit | 711-175-152 | 免疫荧光二抗 | Jackson | 1∶500-1000 |
| Donkey anti-Mouse | 715-175-150 | 免疫荧光二抗 | Jackson | 1∶500-1000 |
Atto 647N | Goat anti Mouse | 50185 | 免疫荧光二抗 | Sigma | 1∶500-1000 |
Blue | DAPI (single-stranded)(5mg/ml) | D-9564 | 单链DNA荧光染料 | Sigma | 1∶1000 |
| DAPI (double-stranded)(5mg/ml) | D-8417 | 双链DNA荧光染料 | Sigma | 1∶1000 |
| Hoechst 33258(5mg/ml) | B1155 | 活细胞DNA荧光染料 | Sigma | 1∶1000 |
Red | PI (2.5mg/ml) | P4170 | 核苷酸荧光染料 | Sigma | 1∶1000 |
| LysoTracker | L-7528 | 活细胞溶酶体染料 | Molecular Probes | 1∶2000-5000 |
| MitoTracker | M-7510 | 活细胞线粒体染料 | Molecular Probes | 1∶10000-40000 |
| Phalloidin Alexa Fluor 555 | A34055 | F-actin荧光染料 | Molecular Probes | 1∶100 |
| Phalloidin TRITC | P1951 | F-actin荧光染料 | Sigma | 1∶1000 |
Green | Fluo 3-AM(100μM) | F023 | 钙离子荧光探针 | DOJINDO | |
| Goat anti-Mouse HRP | PB001 | 免疫酶标二抗 | 上海生科院抗体中心 | 1∶100 |
| Goat anti-Rabbit HRP | PB003 | 免疫酶标二抗 | 上海生科院抗体中心 | 1∶100 |
| Metal Enhanced DAB Substrate Kit | | HRP底物 | Thermo | |
| | | | | |
| Monoclone anti α-tubulin | T-5168 | α-tubulin抗体 | Sigma | 1∶1000 |
| Monoclone anti Golgi GM130 | 610823 | 顺式高尔基抗体 | BD | 1∶500 |
| Anti-Calnexin (produced in rabbit) | C4731 | 内质网抗体 | Sigma | 1∶500 |
| EEA1 | 610457 | 早期内涵体抗体 | BD | 1∶500 |
| Monoclone anti-Flag | F3165 | Flag抗体 | Sigma | |
| Mouse IgG (2mg/ml) | I5381 | 小鼠免疫球蛋白G | Sigma | |
| Rabbit IgG (1mg/ml) | I5006 | 兔免疫球蛋白G | Sigma | |
| Donkey serum | S30-100mL | 驴血清 | Chemico | |
| Mounting Medium: Mowiol® 4-88 | 81381 | 荧光封片剂 | Sigma | |
| Paraformaldehyde (PFA) | P6148 | 组织细胞固定液 | Sigma | |
| HE 染液 | | 组织细胞结构染色 | 国产 | |
细胞分析技术常用耗材及来源
耗材名称 | 规格 | 货号 | 用途 | 来源 | 包装单位 |
盖玻片(0.13μm-0.17μm) | 圆:12mm, 25mm | | 细胞培养 | Fisher | |
盖玻片(0.13μm-0.17μm) | 方:10mm×10mm, 22mm×22mm, 24mm×30mm, 24mm×50mm 圆:12mm, 25mm | | 细胞培养及封片 | 国产 | 100片/盒 |
APES处理载玻片 | | | 切片贴片用 | 国产 | 50片/盒 |
多聚赖氨酸处理玻片 | | | 切片贴片用 | 国产 | 50片/盒 |
滤网cell strainer | 40μm | 352340 | 过滤细胞 | BD | 个 |
70μm | 352350 |
流式管 (FACS Tube) | No cap. Non-sterile. | 352008 | 流式细胞仪 | BD | 个 |
With dual-position snap cap. Sterile. | 352054 |
流式管 | | | 流式细胞仪 | 国产 | 个 |
Glass bottom culture dishes | dish:35μm; microwell diameter:10μm | P35G-1.0-14-C | 共聚焦观察活细胞玻璃底培养皿 | MatTek corporation | 10个/包 |
dish:35μm; microwell diameter:20μm | | 共聚焦观察活细胞玻璃底培养皿 | 国产 | 10个/包 |
Disposable blades 819 | | 14035838925 | 组织切片机一次性刀片 | Leica | 片 |
Tissue Freezing Medium(OCT) | | 14020108926 | 冰冻切片包埋剂 | Leica | 120ml/瓶 |
HistoMold | 6x8μm | 14702218311 | 冰冻切片包埋盒 | Leica | 个 |
13x19μm | 14702218313 |
Cassettes | | | 石蜡包埋筐 | Leica | 个 |
Embedding molds | | | 石蜡包埋筐 | Leica | 个 |
Embedding Rings | | 22-038197 | 石蜡包埋环 | Fisher | 个 |
Disposable Base Mold | 7x7x5μm | 15154-631 | 石蜡包埋盒 | VWR | 个 |
15x15x5μm | 15154-642 |
24x24x5μm | 15154-653 |
30x24x5μm | 15154-664 |
Tissue Capsule | 29x6μm | 15-182-219 | 石蜡包埋筐(圆形) | Fisher | 个 |
38x10μm | 15-182-218 |