microscopic photography and computer image analysis
1 introduce imaging device: fluorescence microscope and computer.
2 teach photography process
① Open the bright field power, choose bright field light path channel.
② Open the ACT-1.
③ Place the slide on the stage.
④ Adjust the right lighting source and exposure time.
⑤ Choose the right picture pixels and save format in the “image” option.
⑥ Select or create a folder in the ”save” option, input the image name in the “prefix” option.
⑦ Click the “exposure” after adjusting the focal length to make image clearly.
3 teach software analysis method.
① Open the morphological analysis software.
② Open the image to be analysed.
③ Click the Color image segmentation in the “manage” option.
④ Drag the mouse to choose the target cell.
⑤ Click the “caculate” and “statistics”.
⑥ Send the result to the word and save.